The assay used will probably illustrate the relationship between the amount of fluorescence generated and the concentration of the target, allowing you to convert simply by dividing by a constant. For example, you may measure the fluorescence of a sample, but would like to plot in concentration units. Additionally, Y values can be transformed into different units by multiplying or dividing by the appropriate constant. Said response can result in a downward sigmoidal curve or an upward sigmoidal curve, representing the effect that the drug has on the system. But before we do that, let us dive a little deeper into the theory behind this analysis.Īs mentioned before, Y values are the responses measured at that dose (concentration) of the drug in question. Once the experiments have been performed, the data obtained and the curve plotted, a plethora of statistical analyses can be ran on to obtain greater insight on study, like the famous EC50 and IC50 values. Plotting these value usually results in a sigmoidal curve (or the dose-response curve) with a bottom and a top flat section, or “plateaus”. In a dose-response curve, the X values represent the drug concentrations or a function of concentrations (like the logarithm of concentrations) and the Y values represent the response that is under question. Today we will learn some of the basic concepts of Dose-Dependent responses, as well as a simple way to graph and analyze them using the GraphPad Prism software. Fortunately, this mathematical model is one of GraphPad Prism’s specialty. But even a complex situation like this can be analyzed with the relatively simple approach of plotting X against Y values. 圖文：康恩宇(Robert Midence)/生醫所博士生 From a pharmacological perspective, the most classic version of a dose-response experiment would be the determining of a drugs effect on a biological system, like cells grown in vitro.

Ic50 graphpad prism 5